Parisa Yazdanshenas; Raana Jahanbin; Abdoullah Mohammadi Sangcheshmeh; Mehdi Aminafshar; Hossein Vaseghi Dodaran; Hamid Varnaseri; Mohammad Chamani; Mohammad Hassan Nazaran
Volume 18, Issue 1 , April 2016, , Pages 173-181
Abstract
This study aimed to investigate the effect of different concentration of zinc nano-complex on bull spermatozoa quality and pregnancy outcome after freeze-thawing process. Ejaculates were collected from four Holstein bulls, twice a week during four weeks. Semen samples diluted with Bioxcell extender containing ...
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This study aimed to investigate the effect of different concentration of zinc nano-complex on bull spermatozoa quality and pregnancy outcome after freeze-thawing process. Ejaculates were collected from four Holstein bulls, twice a week during four weeks. Semen samples diluted with Bioxcell extender containing zero (Control), 10-6, 10-5, 10-4, 10-3 and 10-2 M of zinc nano-complex. The samples were then freezed-thawed after 72 hours. Motility of sperm was analyzed using computer assisted sperm analysis. The highest and lowest concentrations of zinc nano-complex along with control group were analyzed for the DNA fragmentation, malondialdehyde (MDA) concentration, mitochondrial activity, and pregnancy outcomes. The proportion of the total and progressive motile spermatozoa and the percentage of spermatozoa with fragmented DNA were not different among the groups. However, the groups containing 10-6 and 10-2 M zinc nano-complex had a lower level of MDA concentration and higher activity of mitochondria. Moreover, the pregnancy outcome was not affected by the treatment with zinc nano-complex. In conclusion, our data revealed that supplementation of bull semen Bioxcell extender with zinc nano-complex can decrease the level of MDA and improve mitochondrial activity, although such an improvement was not reflected in results of fertility rates.